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KMID : 0359319720120010097
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Korean Journal of Veterinary Research
1972 Volume.12 No. 1 p.97 ~ p.119
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Studies on Modified Complement Fixation Test of Bovine Blackleg
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Abstract
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Studies on modified complement fixation (MCF) test of antiblackleg bovine serum were made and the results obtained were summarized as followings.
1. The most satisfactory antigen for the MCF test among various materials studied was found to be the vegetative cells of Cl. chauvoei grown in cooked meat medium (CMM) containing 0.5mM l-(alpha) alanine and 0.1mM manganese. The antigen was prepared by inoculating the spores of Cl. chauvoei, heated at 70˚C. for 30 minutes, into the (CMM followed by incubation at 37℃. for 15 hours.
2. An active component contained in the factor serum of fresh normal rabbit serum was found to be C´4 fraction. It was also shown that, furthermore, DEAE cellulose sieved C’S fraction of the factor serum enhanced antibody titer and the highest antibody titer was resulted by the addition of 0.03㎖. of the factor serum to each tube.
3. More than four fold increases of antibody titer, in antiblbckleg bovine serum-antigen system, was made with the MCF test than that with the direct complement fixation test.
4. The MCF antibody titer of cattle vaccinated against blackleg was 128 until seven month and 64 for five months thereafter.
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